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DNA methylation assay To test
2019-08-01
DNA methylation assay. To test the biological activity of purified A1S_0222, a methylation assay was performed using the Int1 DNA. As an alternative DNA substrate, Seq3 (located in gene A1S_0965 of A. baumannii ATCC 17978) was amplified from Acinetobacter baumannii 29D2 using the oligonucleotides Se
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In the present study we implemented the
2019-08-01
In the present study, we implemented the dendritic RCA-induced formation of amplifying DNAzyme catalytic labels strategy for sensitive and accurate assay of MTase activity and the detection of DNA methylation. In comparison with the traditional RCA process, the proposed strategy achieved high amplif
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Cell migration and invasion are
2019-08-01
Cell migration and invasion are central processes in the development and metastasis of cancer. The regulation of cell migration by DDR1 has been reported in many types of cancer cell lines; however, conflicting results have been reported regarding the inhibitory as well as promotive effects of DDR1
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E protein expressed in a plasmid encoding codon
2019-07-31
E1 protein expressed in a plasmid encoding codon-optimized canine oral papillomavirus (COPV) led to therapeutic immunization of COPV-infected Beagle dogs [25], [26], which was better than that achieved with plasmids encoding E2 or E7 COPV. Moreover, E1 and E2 from cottontail rabbit papillomavirus (C
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Common enzyme quantitative methods include enzyme linked imm
2019-07-31
Common enzyme quantitative methods include enzyme-linked immunosorbent assay [,] (ELISA), chemiluminescence (CLIA) [, , ] and immunochromatography []. The advantages of ELISA are cost reduction and automation, but non-specific reaction caused by HOOK effect, false positive caused by cross-contaminat
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Hedamycin isolated from Streptomyces griseoruber belongs to
2019-07-31
Hedamycin, isolated from Streptomyces griseoruber, belongs to the highly cytotoxic pluramycin class of Guvacoline hydrobromide where (Figure 3(c)). It consists of a planar anthrapyrantrione chromophore attached to two amino sugar rings at one end and a bisepoxide-containing side-chain at the other e
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During host cell interactions G intestinalis
2019-07-31
During host-cell interactions, G. intestinalis trophozoites up-regulate several cell-associated proteins, including high-cysteine membrane proteins (HCMPs) and variant surface proteins (VSPs) [8]. The parasite also releases around 200 proteins upon host-cell contact, including many proteases [9,10].
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To elucidate the potential mechanism underlying osteosarcoma
2019-07-31
To elucidate the potential mechanism underlying osteosarcoma cell growth decrease by CDK4 inhibition, flow cytometry analysis was used to determine NS3694 to and apoptosis in human osteosarcoma cells after palbociclib treatment. The results showed that osteosarcoma cells were arrested in G1 phase o
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Introduction Cyclin dependent kinases CDKs are a
2019-07-31
Introduction Cyclin dependent kinases (CDKs) are a family of proline-directed serine/threonine kinases with roles in regulation of crucial cellular processes such as Cefotaxime sodium salt and transcription [1]. One of the unifying features of this family is its dependence on association with cycl
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Accurate equilibrium predictions of CO containing
2019-07-31
Accurate equilibrium predictions of CO2 containing mixtures, however, are challenging with traditional equations of state. The reason for this may be that CO2 has a large quadrupole moment. The large quadrupole moment of CO2, as well as size asymmetry, are for instance believed to be the reasons for
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Future analysis could consider using fixed effects also with
2019-07-31
Future analysis could consider using fixed effects also with the level set field, , in order to investigate which covariates explain the classification, a feature of the proposed model that we have not yet investigated. Further, multi-type point patterns may also be analyzed with the proposed model
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In the present study we investigated the effect of
2019-07-31
In the present study we investigated the effect of morphine withdrawal on G-protein coupling to opioid and ET receptors in neonatal rats. Morphine-induced G-protein activation was significantly higher in neonatal rats undergoing morphine withdrawal; suggesting enhanced coupling of the receptors to G
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In normal chow fed mice EP deficiency also decreased the
2019-07-31
In normal chow fed mice, EP4 deficiency also decreased the expression of CYP8B1, the downstream target of CYP7A1. Similarly, knockdown of EP4 with small interfering RNA reduced the expression of CYP8B1 in HepG2 cells. Therefore, it was anticipated that there would be an increased expression of CYP8B
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We propose that unlike other autophagy
2019-07-31
We propose that, unlike other autophagy genes, the specific increase in the expression of IRGM by pEIF2A plays a major role as a checkpoint allowing host cell survival by autophagy or host cell elimination by necroptosis. The localization of IRGM to the mitochondrial inner membrane, its ability to i
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Introduction Cognitive impairment is a core stable
2019-07-31
Introduction Cognitive impairment is a core, stable feature of schizophrenia that limits patient functioning and well-being (Keefe and Harvey, 2012) and associates with poor functional outcome (Fervaha et al., 2014, Kontaxaki et al., 2014). Studies also indicate that cognitive deficits occur prior
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